RT Journal Article
SR Electronic
T1 A Survey of Genome Editing Activity for 16 Cpf1 orthologs
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 134015
DO 10.1101/134015
A1 Bernd Zetsche
A1 Jonathan Strecker
A1 Omar O. Abudayyeh
A1 Jonathan S. Gootenberg
A1 David A. Scott
A1 Feng Zhang
YR 2017
UL http://biorxiv.org/content/early/2017/05/04/134015.abstract
AB The recently discovered class 2 CRISPR-Cas endonuclease Cpf1 offers several advantages over Cas9, including the ability to process its own array and requirement for just a single RNA guide. These attributes make Cpf1 promising for many genome engineering applications. To further expand the suite of Cpf1 tools available, we tested 16 Cpf1 orthologs for activity in eukaryotic cells. Four of these new enzymes demonstrated targeted activity, one of which, from Moraxella bovoculi AAX11_00205 (Mb3Cpf1), exhibited robust indel formation. We also show that Mb3Cpf1 displays some tolerance for a shortened PAM (TTN versus the canonical Cpf1 PAM TTTV). The addition of these enzymes to the genome editing toolbox will further expand the utility of this powerful technology.