RT Journal Article
SR Electronic
T1 Tumor-infiltrating immune repertoires captured by single-cell barcoding in emulsion
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 134841
DO 10.1101/134841
A1 Adrian W. Briggs
A1 Stephen J. Goldfless
A1 Sonia Timberlake
A1 Brian J. Belmont
A1 Christopher R. Clouser
A1 David Koppstein
A1 Devin Sok
A1 Jason Vander A. Heiden
A1 Manu V. Tamminen
A1 Steven H. Kleinstein
A1 Dennis R. Burton
A1 George M. Church
A1 Francois Vigneault
YR 2017
UL http://biorxiv.org/content/early/2017/05/05/134841.abstract
AB Tumor-infiltrating lymphocytes (TILs) are critical to anti-cancer immune responses, but their diverse phenotypes and functions remain poorly understood and challenging to study. We therefore developed a single-cell barcoding technology for deep characterization of TILs without the need for cell-sorting or culture. Our emulsion-based method captures full-length, natively paired B-cell and T-cell receptor (BCR and TCR) sequences from lymphocytes among millions of input cells. We validated the method with 3 million B-cells from healthy human blood and 350,000 B-cells from an HIV elite controller, before processing 400,000 cells from an unsorted dissociated ovarian adenocarcinoma and recovering paired BCRs and TCRs from over 11,000 TILs. We then extended the barcoding method to detect DNA-labeled antibodies, allowing ultra-high throughput, simultaneous protein detection and RNA sequencing from single cells.