PT - JOURNAL ARTICLE AU - Anna-Karin Gustavsson AU - Petar N. Petrov AU - Maurice Y. Lee AU - Yoav Shechtman AU - W. E. Moerner TI - 3D single-molecule super-resolution microscopy with a tilted light sheet AID - 10.1101/135699 DP - 2017 Jan 01 TA - bioRxiv PG - 135699 4099 - http://biorxiv.org/content/early/2017/05/09/135699.short 4100 - http://biorxiv.org/content/early/2017/05/09/135699.full AB - Tilted light sheet microscopy with 3D point spread functions (TILT3D) combines a tilted light sheet illumination scheme and engineered point spread functions with axial ranges up to ten μm for low background, 3D super-localization of single molecules. This system is built upon a standard inverted microscope and has minimal custom parts. The result is simple and flexible 3D super-resolution imaging with tens of nm localization precision throughout thick mammalian cells.