PT  - JOURNAL ARTICLE
AU  - Doyeon Kim
AU  - Nuri Oh
AU  - Kyoohyun Kim
AU  - SangYun Lee
AU  - Chan-Gi Pack
AU  - Ji-Ho Park
AU  - YongKeun Park
TI  - Label-free high-resolution 3-D imaging of gold nanoparticles inside live cells using optical diffraction tomography
AID  - 10.1101/097113
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 097113
4099  - http://biorxiv.org/content/early/2017/05/22/097113.short
4100  - http://biorxiv.org/content/early/2017/05/22/097113.full
AB  - Delivery of gold nanoparticles (GNPs) into live cells has high potentials, ranging from molecular-specific imaging, photodiagnostics, to photothermal therapy. However, studying the long-term dynamics of cells with GNPs using conventional fluorescence techniques suffers from phototoxicity and photobleaching. Here, we present a method for 3-D imaging of GNPs inside live cells exploiting refractive index (RI) as imaging contrast. Employing optical diffraction tomography, 3-D RI tomograms of live cells with GNPs are precisely measured for an extended period with sub-micrometer resolution. The locations and contents of GNPs in live cells are precisely addressed and quantified due to their distinctly high RI values, which was validated by confocal fluorescence imaging of fluorescent dye conjugated GNPs. In addition, we perform quantitative imaging analysis including the segmentations of GNPs in the cytosol, the volume distributions of aggregated GNPs, and the temporal evolution of GNPs contents in HeLa and 4T1 cells.GNPsgold nanoparticlesRIrefractive indexODToptical diffraction tomographyDMDdigital micromirror device