@article {Wang145870, author = {Xuxu Wang and Xiling Fu and Min Chen and Lei Huan and Wenhai Liu and Yangang Gao and Wei Xiao and Xiude Chen and Ling Li and Dongsheng Gao}, title = {Sugar metabolism changes in response to the ultraviolet B irradiation of peach (Prunus persica L.)}, elocation-id = {145870}, year = {2017}, doi = {10.1101/145870}, publisher = {Cold Spring Harbor Laboratory}, abstract = {The protected cultivation of peach (Prunuspersica L.) trees is more economical and efficient than traditional cultivation, resulting in increased farmers{\textquoteright} incomes, but the peach sugar contents are lower than in open planting. In the greenhouse, a high-sugar variety of peach {\textquoteleft}Lumi 1{\textquoteright} was irradiated with 1.44 KJ{\textperiodcentered}m-2.d-1 intensity ultraviolet B radiation. The soluble sugar contents in fruit, peel and leaf were quantified using liquid chromatography. Overall, sucrose and sorbitol increased before the second fruit-expansion period. To further understand the mechanisms regulating sucrose and sorbitol accumulation in peach fruit, expression profiles of genes involved in sugar metabolism and transport were measured. The activity and translocation protein contents of these enzymes were measured by enzyme-linked immunosorbent assay. The increased sucrose synthase activity and sucrose transporter level in the pericarp promoted the synthesis of sucrose and intake of sucrose into fruit. Sorbitol transport into fruit was promoted by the increased sorbitol transporter protein levels in leaves. In summary, greenhouse the sucrose and sorbitol contents were increased when supplemented with 1.44 kJ{\textperiodcentered}m-2{\textperiodcentered}d-1 ultraviolet B radiation before the second fruit-expansion period of peach.}, URL = {https://www.biorxiv.org/content/early/2017/06/04/145870}, eprint = {https://www.biorxiv.org/content/early/2017/06/04/145870.full.pdf}, journal = {bioRxiv} }