RT Journal Article
SR Electronic
T1 Genome-Wide Association Study of Ionomic Traits on Diverse Soybean Populations from Germplasm Collections
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 079731
DO 10.1101/079731
A1 Greg Ziegler
A1 Randall Nelson
A1 Stephanie Granada
A1 Hari B. Krishnan
A1 Jason D. Gillman
A1 Ivan Baxter
YR 2017
UL http://biorxiv.org/content/early/2017/06/04/079731.abstract
AB The elemental content of a soybean seed is a determined by both genetic and environmental factors and is an important component of its nutritional value. The elemental content is chemically stable, making the samples stored in germplasm repositories an intriguing source of experimental material. To test the efficacy of using samples from germplasm banks for gene discovery, we analyzed the elemental profile of seeds from 1653 lines in the USDA Soybean Germplasm Collection. We observed large differences in the elemental profiles based on where the lines were grown, which lead us to break up the genetic analysis into multiple small experiments. Despite these challenges, we were able to identify candidate SNPs controlling elemental accumulation as well as lines with extreme elemental accumulation phenotypes. Our results suggest that elemental analysis of germplasm samples can identify SNPs in linkage disequilibrium to genes, which can be leveraged to assist in crop improvement efforts.