PT  - JOURNAL ARTICLE
AU  - Yan Li
AU  - Jiafeng Yuan
AU  - Qianwen Wang
AU  - Lijie Sun
AU  - Yunying Sha
AU  - Yanxiang Li
AU  - Lizhong Wang
AU  - Zhonghua Wang
TI  - The collective influence of 1, 25-dihydroxyvitamin D<sub>3</sub> with physiological fluid shear stress on osteoblasts
AID  - 10.1101/146159
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 146159
4099  - http://biorxiv.org/content/early/2017/06/05/146159.short
4100  - http://biorxiv.org/content/early/2017/06/05/146159.full
AB  - 1, 25-dihydroxyvitamin D3 (1, 25 (OH)2 D3) and mechanical stimuli in physiological environment play an important role in the pathogenesis of osteoporosis. The effects of 1, 25-dihydroxyvitamin D3 alone and mechanical stimuli alone on osteoblasts have been widely investigated. This study reports the collective influences of 1, 25-dihydroxyvitamin D3 and flow shear stress (FSS) on biological functions of osteoblasts. 1, 25 (OH)2 D3 were constructed in various kinds of concentration (0, 1, 10, 100 nmmol/L), while physiological fluid shear stress (12 dynes/cm2) were produced by using a parallel-plate fluid flow system. 1, 25 (OH)2 D3 affects the responses of ROBs to FSS, including the inhibition of NO releases and cell proliferation as well as the promotion of PGE2 releases and cell differentiation. These findings provide a possible mechanism by which 1, 25(OH)2 D3 influences osteoblasts responses to FSS and may provide guidance for the selection of 1, 25(OH)2 D3 concentration and mechanical loading in order to in vitro produce functional bone tissues.