RT Journal Article
SR Electronic
T1 Cell type boundaries organize plant development
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 126466
DO 10.1101/126466
A1 Monica Pia Caggiano
A1 Xiulian Yu
A1 Neha Bhatia
A1 André Larsson
A1 Hasthi Ram
A1 Carolyn Ohno
A1 Pia Sappl
A1 Elliot M. Meyerowitz
A1 Henrik Jönsson
A1 Marcus G. Heisler
YR 2017
UL http://biorxiv.org/content/early/2017/06/12/126466.abstract
AB In plants the dorsoventral boundary of leaves defines an axis of symmetry through the centre of the organ separating the top (dorsal) and bottom (ventral) tissues. Although the positioning of this boundary is critical for leaf morphogenesis, how the boundary is established and how it influences development remains unclear. Using live-imaging and perturbation experiments we show that leaf orientation, morphology and position are pre-patterned by HD-ZIPIII and KAN gene expression in the shoot, leading to a model in which dorsoventral genes coordinate to regulate plant development by localizing auxin response between their expression domains. However we also find that auxin levels feedback on dorsoventral patterning by spatially organizing HD-ZIPIII and KAN expression in the shoot periphery. By demonstrating that the regulation of these genes by auxin also governs their response to wounds, our results also provide a parsimonious explanation for the influence of wounds on leaf dorsoventrality.