@article {Lou134775, author = {Hong Lou and Eduardo Gharzouzi and Sarita Polo Guerra and Jo{\"e}l Fokom Domgue and Julie Sawitzke and Guillermo Villagran and Lisa Garland and Joseph Boland and Sarah Wagner and Joanna Martinez and H{\'e}ctor Rosas and Jami Troxler and Heidi McMillen and Bailey Kessing and Enrique Alvirez and Miriam Castillo and Hesler Morales and Victor Argueta and Andert Rosingh and Femke J.H.B. van Aerde-van Nunen and Griselda Lopez and Mark Schiffman and Michael Dean and Roberto Orozco}, title = {Low-cost HPV testing and the prevalence of cervical infection in asymptomatic populations in Guatemala}, elocation-id = {134775}, year = {2017}, doi = {10.1101/134775}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Background A low cost and accurate method for detecting high-risk (HR) HPV is important to permit HPV testing for cervical cancer prevention. We validated a low-cost commercially available HPV method (H13, Hybribio, Hong Kong) and determined the distribution of HPV infections in over 1717 cancer-free women in Guatemala.Methods H13 results were compared with two more established HPV tests: (Xpert{\texttrademark} (Cepheid) and SPF10-LIPA25{\texttrademark} (DDL)) in 40 mainly known positive specimens. HR-HPV was detected in cervical samples from 1717 cancer-free women receiving Pap smears using the Hybribio{\texttrademark} real-time PCR assay of 13 HR types. Selected HPV positive samples were sequenced to determine viral type.Results The Hybribio H13 Assay showed 93\% identical results with Xpert, and 89\% with SPF1 0-LIPA25. A total of 13\% (226/1717) of women tested HPV+. The highest prevalence was found in younger women (\<30 years, 22 \%) and older ones (>= 60 years, 15\%). The six most common HR-HPV types among the 148 HPV+ typed were HPV16 (22\%), HPV18 (11\%), HPV39 (11\%), HPV58 (10\%), HPV52 (8\%), and HPV45 (8\%).Conclusions The Hybribio Assay was low cost, and accurate in testing for HR-HPV infection. As in most places, HPV16 was the most prevalent HR type in Guatemala and the age-specific prevalence curve peaked in younger ages.A low cost and accurate method, Hybribio Assay, could be used for CC testing in low income regions.A total of 13\% of cancer-free women were HPV+ and positivity was associated with younger age (\<30 years old) in Guatemala.HPV16 was the major prevalent type.}, URL = {https://www.biorxiv.org/content/early/2017/06/13/134775}, eprint = {https://www.biorxiv.org/content/early/2017/06/13/134775.full.pdf}, journal = {bioRxiv} }