RT Journal Article SR Electronic T1 A reagentless biosensor for mRNA: a new tool to study transcription JF bioRxiv FD Cold Spring Harbor Laboratory SP 142794 DO 10.1101/142794 A1 Alexander Cook A1 Yukti Hari-Gupta A1 Christopher P. Toseland YR 2017 UL http://biorxiv.org/content/early/2017/06/15/142794.abstract AB Gene expression, catalysed by RNA polymerases, is one of the most fundamental processes in living cells. Yet, the means to study their activity are currently limited. The majority of methods to quantify mRNA are based upon initial purification of the nucleic acid. This leads to experimental inaccuracies and loss of product. Here, we describe the use of a reagentless mRNA fluorescent biosensor based upon the single stranded binding (SSB) protein. In this study, SSB showed similar binding properties to mRNA, to that of its native substrate, ssDNA. Furthermore, fluorescently labelled MDCC-SSB gave the same fluorescence response with both ssDNA and ssRNA, in a concentration dependent manner. When directly compared to RT-qPCR, we found the biosensor to be more reproducible with no product lost through purification. Therefore, the MDCC-SSB is a novel tool for comparative measurement of mRNA yield following in vitro transcription.