RT Journal Article
SR Electronic
T1 High-quality <em>de novo</em> genome assembly of the <em>Dekkera bruxellensis</em> UMY321 yeast isolate using Nanopore MinION sequencing
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 151167
DO 10.1101/151167
A1 Téo Fournier
A1 Jean-Sébastien Gounot
A1 Kelle Freel
A1 Corinne Cruaud
A1 Arnaud Lemainque
A1 Jean-Marc Aury
A1 Patrick Wincker
A1 Joseph Schacherer
A1 Anne Friedrich
YR 2017
UL http://biorxiv.org/content/early/2017/06/16/151167.abstract
AB Genetic variation in natural populations represents the raw material for phenotypic diversity. Species-wide characterization of genetic variants is crucial to have a deeper insight into the genotype-phenotype relationship. With the advent of new sequencing strategies and more recently the release of long-read sequencing platforms, it is now possible to explore the genetic diversity of any non-model organisms, representing a fundamental resource for biological research. In the frame of population genomic surveys, a first step is evidently to obtain the complete sequence and high quality assembly of a reference genome. Here, we completely sequenced and assembled a reference genome of the non-conventional Dekkera bruxellensis yeast. While this species is a major cause of wine spoilage, it paradoxically contributes to the specific flavor profile of some Belgium beers. In addition, an extreme karyotype variability is observed across natural isolates, highlighting that D. bruxellensis genome is very dynamic. The whole genome of the D. bruxellensis UMY321 isolate was sequenced using a combination of Nanopore long-read and Illumina short-read sequencing data. We generated the most complete and contiguous de novo assembly of D. bruxellensis to date and obtained a first glimpse into the genomic variability within this species by comparing the sequences of several isolates. This genome sequence is therefore of high value for population genomic surveys and represents a reference to study genome dynamic in this yeast species.