RT Journal Article
SR Electronic
T1 A practical guide to CRISPR/Cas9 genome editing in Lepidoptera
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 130344
DO 10.1101/130344
A1 Linlin Zhang
A1 Robert D. Reed
YR 2017
UL http://biorxiv.org/content/early/2017/06/22/130344.abstract
AB CRISPR/Cas9 genome editing has revolutionized functional genetic work in many organisms and is having an especially strong impact in emerging model systems. Here we summarize recent advances in applying CRISPR/Cas9 methods in Lepidoptera, with a focus on providing practical advice on the entire process of genome editing from experimental design through to genotyping. We also describe successful targeted GFP knockins that we have achieved in butterflies. Finally, we provide a complete, detailed protocol for producing targeted long deletions in butterflies.