Multiplex staining by sequential immunostaining and antibody removal on routine tissue sections

MM Bolognesi, M Manzoni, CR Scalia… - … of Histochemistry & …, 2017 - journals.sagepub.com
Multiplexing, labeling for multiple immunostains in the very same cell or tissue section in situ,
has raised considerable interest. The methods proposed include the use of labeled primary …

Antigen masking during fixation and embedding, dissected

CR Scalia, G Boi, MM Bolognesi… - … of Histochemistry & …, 2017 - journals.sagepub.com
Antigen masking in routinely processed tissue is a poorly understood process caused by
multiple factors. We sought to dissect the effect on antigenicity of each step of processing by …

Elution of High-affinity (>10-9 KD) Antibodies from Tissue Sections: Clues to the Molecular Mechanism and Use in Sequential Immunostaining

R Gendusa, CR Scalia, S Buscone… - … of Histochemistry & …, 2014 - journals.sagepub.com
Inconsistent results obtained with published methods for the elution of antibodies from
tissue sections prompted the assessment of both old and new methods in combination with …

Disaccharides protect antigens from drying-induced damage in routinely processed tissue sections

G Boi, CR Scalia, R Gendusa… - … of Histochemistry & …, 2016 - journals.sagepub.com
… The authors disclosed receipt of the following financial support for the research, authorship,
and/or publication of this article: Rossella Gendusa and Carla Rossana Scalia’s salary are …

Epitope recognition in the Human–Pig comparison model on fixed and embedded material

CR Scalia, R Gendusa, M Basciu… - … of Histochemistry & …, 2015 - journals.sagepub.com
… The authors disclosed receipt of the following financial support for the research, authorship,
and/or publication of this article: Carla Rossana Scalia, Rossella Gendusa and Maddalena …

[HTML][HTML] Economic assessment of NGS testing workflow for NSCLC in a healthcare setting

…, V L'Imperio, G Casati, J Ceku, D Pilla, CR Scalia… - Heliyon, 2024 - cell.com
Background . The molecular diagnostic and therapeutic pathway of Non-Small Cell Lung
Cancer (NSCLC) stands as a successful example of precision medicine. The scarcity of …

Double autoimmunostaining with glycine treatment

K Hasui, T Takatsuka, R Sakamoto… - … of Histochemistry & …, 2003 - journals.sagepub.com
Double autoimmunostaining by a sequential twice-repeated enzyme-labeled polymer
method was examined on archival paraffin sections of formalin-fixed human tissue using an …

Epitopes of components of the plasminogen activation system are re-exposed in formalin-fixed paraffin sections by different retrieval techniques

CM Ferrier, WL van Geloof… - … of Histochemistry & …, 1998 - journals.sagepub.com
We present a systematic analysis of the sensitivity and specificity of immunohistochemical
stainings for components of the plasminogen activation system, ie, uPA, tPA, PAI-1, PAI-2, and …

Localization and Identification of Schistosoma mansoni/KLH-crossreactive Components in Infected Mice

C Thors, E Linder - Journal of Histochemistry & …, 2003 - journals.sagepub.com
KLH (Keyhole limpet hemocyanin) is highly immunogenic, and crossreactive epitopes occur
widely in nature. In schistosomiasis, infected hosts generate antibodies reactive with KLH. …

Comparative analysis of an improved thioflavin-s stain, Gallyas silver stain, and immunohistochemistry for neurofibrillary tangle demonstration on the same sections

A Sun, XV Nguyen, G Bing - Journal of Histochemistry & …, 2002 - journals.sagepub.com
An improved thioflavin-S stain, Gallyas silver stain, and two immunostainings were quantitatively
compared for demonstration of neurofibrillary tangles (NFTs) on the same sections. …