Tissues derive ATP from two pathways—glycolysis and the tricarboxylic acid (TCA) cycle
coupled to the electron transport chain. Most energy in mammals is produced via TCA …

Decreased NAD + levels have been shown to contribute to metabolic dysfunction during
aging. NAD + decline can be partially prevented by knockout of the enzyme CD38. However, it …

10.7554/eLife.28899.001 Here we report multiple lines of evidence for a comprehensive model
of energy metabolism in the vertebrate eye. Metabolic flux, locations of key enzymes, and …

Glycolysis plays a central role in organismal metabolism, but its quantitative inputs across
mammalian tissues remain unclear. Here we use 13 C-tracing in mice to quantify glycolytic …

Nicotinamide adenine dinucleotide (NAD) is an essential redox cofactor in mammals and
microbes. Here we use isotope tracing to investigate the precursors supporting NAD synthesis …

The retinal pigment epithelium (RPE) is a monolayer of pigmented cells that requires an
active metabolism to maintain outer retinal homeostasis and compensate for oxidative stress. …

Metabolite transport is a major function of the retinal pigment epithelium (RPE) to support the
neural retina. RPE dysfunction plays a significant role in retinal degenerative diseases. We …

Selenium restriction augments ascorbate efficacy and extends lifespan in a mouse xenograft
model of glioblastoma, suggesting that targeting selenium-mediated antioxidant defenses …

Oxidative stress has been implicated in the pathogenesis of age-related macular degeneration,
the leading cause of blindness in older adults, with retinal pigment epithelium (RPE) …

NAD + is an essential coenzyme for all living cells. NAD + concentrations decline with age,
but whether this reflects impaired production or accelerated consumption remains unclear. …