The formin Delphilin binds the glutamate receptor, GluRδ2, in dendritic spines of Purkinje
cells. Both proteins play a role in learning. To understand how Delphilin functions in neurons, …

Boussina et al. recently evaluated a deep learning sepsis prediction model (COMPOSER) in
a prospective before-and-after quasi-experimental study within two emergency departments …

… 2B, C, and D were purified by nickel affinity chromatography, eluted, and further purified by
… GST fusions with a C-terminal Cys-His6 tag and purified by nickel affinity chromatography, …

… nickel affinity chromatography based on a flush C-terminal His6 tag. Wild type UBE2C was
purified by SEC in 20 mM HEPES pH 8.0, 200 mM NaCl, 1 mM DTT. The UBE2C~UB oxyester…

The formin Delphilin binds the glutamate receptor, GluRδ2, in dendritic spines of Purkinje
cells. Both proteins play a role in learning. To understand how Delphilin functions in neurons, …

… ; His-BCL-xLΔC was then isolated using nickel affinity beads. His-BCL-xLΔC and associated
… (40 nM), followed by fractionation and either nickel affinity pulldown of His-BCL-xLΔC, SDS-…

BCL-2 proteins regulate mitochondrial poration in apoptosis initiation. How the pore-forming
BCL-2 Effector BAK is activated remains incompletely understood mechanistically. Here we …

Necroptosis is an important form of lytic cell death triggered by injury and infection, but whether
mixed lineage kinase domain-like (MLKL) is sufficient to execute this pathway is unknown…

… and nickel affinity chromatography. After sizing, cycB-NT* and UbcycB-NT* were FlAsH
labeled using a C-terminal tetra-cysteine site. Other proteins were described previously 37 . …

The effector B cell lymphoma-2 (BCL-2) protein BCL-2 ovarian killer (BOK) induces mitochondrial
outer membrane permeabilization (MOMP) to initiate apoptosis upon inhibition of the …