User profiles for Jennifer A. Doudna
 | Jennifer DoudnaProfessor of Molecular and Cell Biology and Chemistry, UC Berkeley Verified email at berkeley.edu Cited by 130534 |
A programmable dual-RNA–guided DNA endonuclease in adaptive bacterial immunity
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas)
systems provide bacteria and archaea with adaptive immunity against viruses and …
systems provide bacteria and archaea with adaptive immunity against viruses and …
The new frontier of genome engineering with CRISPR-Cas9
JA Doudna, E Charpentier - Science, 2014 - science.org
Background Technologies for making and manipulating DNA have enabled advances in
biology ever since the discovery of the DNA double helix. But introducing site-specific …
biology ever since the discovery of the DNA double helix. But introducing site-specific …
RNA-guided genetic silencing systems in bacteria and archaea
Clustered regularly interspaced short palindromic repeat (CRISPR) are essential components
of nucleic-acid-based adaptive immune systems that are widespread in bacteria and …
of nucleic-acid-based adaptive immune systems that are widespread in bacteria and …
Programmable RNA recognition and cleavage by CRISPR/Cas9
The CRISPR-associated protein Cas9 is an RNA-guided DNA endonuclease that uses RNA–DNA
complementarity to identify target sites for sequence-specific double-stranded DNA (…
complementarity to identify target sites for sequence-specific double-stranded DNA (…
CRISPR-Cas guides the future of genetic engineering
… We thank members of the Doudna laboratory for discussion. Special thanks to SC Strutt, JC
… We thank members of the Doudna laboratory for discussion. Special thanks to SC Strutt, JC …
… We thank members of the Doudna laboratory for discussion. Special thanks to SC Strutt, JC …
Conformational control of DNA target cleavage by CRISPR–Cas9
Cas9 is an RNA-guided DNA endonuclease that targets foreign DNA for destruction as part
of a bacterial adaptive immune system mediated by clustered regularly interspaced short …
of a bacterial adaptive immune system mediated by clustered regularly interspaced short …
A Cas9–guide RNA complex preorganized for target DNA recognition
Bacterial adaptive immunity uses CRISPR (clustered regularly interspaced short palindromic
repeats)–associated (Cas) proteins together with CRISPR transcripts for foreign DNA …
repeats)–associated (Cas) proteins together with CRISPR transcripts for foreign DNA …
CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
CRISPR-Cas12a (Cpf1) proteins are RNA-guided enzymes that bind and cut DNA as
components of bacterial adaptive immune systems. Like CRISPR-Cas9, Cas12a has been …
components of bacterial adaptive immune systems. Like CRISPR-Cas9, Cas12a has been …
Integrase-mediated spacer acquisition during CRISPR–Cas adaptive immunity
Bacteria and archaea insert spacer sequences acquired from foreign DNAs into CRISPR
loci to generate immunological memory. The Escherichia coli Cas1–Cas2 complex mediates …
loci to generate immunological memory. The Escherichia coli Cas1–Cas2 complex mediates …
Structures of Cas9 endonucleases reveal RNA-mediated conformational activation
Introduction Bacteria and archaea defend themselves against invasive DNA using adaptive
immune systems comprising CRISPR (clustered regularly interspaced short palindromic …
immune systems comprising CRISPR (clustered regularly interspaced short palindromic …