The clinical efficacy of epidermal growth factor receptor (EGFR) kinase inhibitors in EGFR-mutant
non-small-cell lung cancer (NSCLC) is limited by the development of drug-resistance …

… He is currently a postdoctoral fellow with John R. Engen at the University of New Mexico.
His research has focused on the analysis of the protein backbone from both a chemical and …

In an effort to find new pharmacological modalities to overcome resistance to ATP-binding-site
inhibitors of Bcr–Abl, we recently reported the discovery of GNF-2, a selective allosteric …

Biologics such as monoclonal antibodies are much more complex than small-molecule
drugs, which raises challenging questions for the development and regulatory evaluation of …

Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful biophysical
technique being increasingly applied to a wide variety of problems. As the HDX-MS community …

Recent technological advances hydrogen exchange MS have led to improvements in the
technique’s ability to analyze the shape and movements of proteins. John Engen of …

Post-translational modifications (PTMs) can have profound effects on protein structure and
protein dynamics and thereby can influence protein function. To understand and connect PTM…

The function, efficacy, and safety of protein biopharmaceuticals are tied to their three-dimensional
structure. The analysis and verification of this higher-order structure are critical in …

The conformational properties of proteins can be probed with hydrogen/deuterium exchange
mass spectrometry (HXMS). In order to maintain the deuterium label during LC/MS analyses…

We report the synthesis of a GDP analogue, SML‐8‐73‐1, and a prodrug derivative, SML‐10‐70‐1,
which are selective, direct‐acting covalent inhibitors of the K‐Ras G12C mutant …