Protrusion of lamellipodia and ruffles requires polymerization of branched actin filaments by
the Arp2/3 complex. Although regulation of Arp2/3 complex activity has been extensively …

Nuclear proteins bind chromatin to execute and regulate genome-templated processes. While
studies of individual nucleosome interactions have suggested that an acidic patch on the …

Cell cycle phase transitions are tightly orchestrated to ensure efficient cell cycle progression
and genome stability. Interrogating these transitions is important for understanding both …

The cell cycle is canonically described as a series of four consecutive phases: G1, S, G2,
and M. In single cells, the duration of each phase varies, but the quantitative laws that govern …

Cell migration depends on the dynamic formation and turnover of cell adhesions and is tightly
controlled by actomyosin contractility and local Ca 2+ signals. The divalent cation channel …

Understanding the organization of the cell cycle has been a longstanding goal in cell biology.
We combined time-lapse microscopy, highly multiplexed single-cell imaging of 48 core cell …

The cellular decision governing the transition between proliferative and arrested states is
crucial to the development and function of every tissue. While the molecular mechanisms that …

Super-resolution microscopy (SRM) allows precise localization of proteins in cellular organelles
and structures, including the actin cytoskeleton. Yet sample preparation protocols for …

We developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method for
acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of a …

10.7554/eLife.23649.001 The urokinase receptor (uPAR) is a glycosylphosphatidylinositol (GPI)-anchored
protein that promotes tissue remodeling, tumor cell adhesion, migration and …