Salting out of proteins using ammonium sulfate precipitation
KC Duong-Ly, SB Gabelli - Methods in enzymology, 2014 - Elsevier
Protein solubility is affected by ions. At low ion concentrations (<0.5 M), protein solubility
increases along with ionic strength. Ions in the solution shield protein molecules from the …
increases along with ionic strength. Ions in the solution shield protein molecules from the …
Conformational analysis of the DFG-out kinase motif and biochemical profiling of structurally validated type II inhibitors
Structural coverage of the human kinome has been steadily increasing over time. The structures
provide valuable insights into the molecular basis of kinase function and also provide a …
provide valuable insights into the molecular basis of kinase function and also provide a …
The human kinome and kinase inhibition
KC Duong‐Ly, JR Peterson - Current protocols in …, 2013 - Wiley Online Library
Protein and lipid kinases play key regulatory roles in a number of biological processes.
Unsurprisingly, activating mutations in kinases have been linked to a number of disorders and …
Unsurprisingly, activating mutations in kinases have been linked to a number of disorders and …
T cell activation triggers reversible inosine-5′-monophosphate dehydrogenase assembly
KC Duong-Ly, YM Kuo, MC Johnson… - Journal of cell …, 2018 - journals.biologists.com
T cell-mediated adaptive immunity requires naïve, unstimulated T cells to transition from a
quiescent metabolic state into a highly proliferative state upon T cell receptor engagement. …
quiescent metabolic state into a highly proliferative state upon T cell receptor engagement. …
The conformation of the pore region of the M2 proton channel depends on lipid bilayer environment
KC Duong‐Ly, V Nanda, WF DeGrado… - Protein …, 2005 - Wiley Online Library
The M2 protein from influenza A virus is a 97‐amino‐acid protein with a single transmembrane
helix that forms proton‐selective channels essential to virus function. The hydrophobic …
helix that forms proton‐selective channels essential to virus function. The hydrophobic …
Reconstituted IMPDH polymers accommodate both catalytically active and inactive conformations
…, AL Burrell, MC Johnson, KC Duong-Ly… - Molecular biology of …, 2017 - Am Soc Cell Biol
Several metabolic enzymes undergo reversible polymerization into macromolecular
assemblies. The function of these assemblies is often unclear, but in some cases they regulate …
assemblies. The function of these assemblies is often unclear, but in some cases they regulate …
Gel filtration chromatography (size exclusion chromatography) of proteins
KC Duong-Ly, SB Gabelli - Methods in enzymology, 2014 - Elsevier
The protocol described here allows the student to construct a standard curve for a gel filtration
column with a separation range of 5–250 kD. The size (hydrodynamic radius) of a protein …
column with a separation range of 5–250 kD. The size (hydrodynamic radius) of a protein …
[PDF][PDF] Kinase inhibitor profiling reveals unexpected opportunities to inhibit disease-associated mutant kinases
KC Duong-Ly, K Devarajan, S Liang, KY Horiuchi… - Cell reports, 2016 - cell.com
Small-molecule kinase inhibitors have typically been designed to inhibit wild-type kinases
rather than the mutant forms that frequently arise in diseases such as cancer. Mutations can …
rather than the mutant forms that frequently arise in diseases such as cancer. Mutations can …
Using ion exchange chromatography to purify a recombinantly expressed protein
KC Duong-Ly, SB Gabelli - Methods in enzymology, 2014 - Elsevier
Ion exchange chromatography (IEX) separates molecules by their surface charge, a property
that can vary vastly between different proteins. There are two types of IEX, cation exhange …
that can vary vastly between different proteins. There are two types of IEX, cation exhange …
Affinity purification of a recombinant protein expressed as a fusion with the maltose-binding protein (MBP) tag
KC Duong-Ly, SB Gabelli - Methods in enzymology, 2015 - Elsevier
… Harvest the cells by centrifugation and freeze the cell pellet at − 80 C (optional). Resuspend
the cell pellet in about 10-ml binding buffer per gram of cells, lyse the cells (eg, by sonication …
the cell pellet in about 10-ml binding buffer per gram of cells, lyse the cells (eg, by sonication …