Fluoropyrimidines are the first-line treatment for colorectal cancer, but their efficacy is highly
variable between patients. We queried whether gut microbes, a known source of inter-…

Microbial communities are composed of cells of varying metabolic capacity, and regularly
include auxotrophs that lack essential metabolic pathways. Through analysis of auxotrophs for …

Nutritional codependence (syntrophy) has underexplored potential to improve
biotechnological processes by using cooperating cell types. So far, design of yeast syntrophic …

Metabolism is deeply intertwined with aging. Effects of metabolic interventions on aging
have been explained with intracellular metabolism, growth control, and signaling. Studying …

Both single and multicellular organisms depend on anti-stress mechanisms that enable them
to deal with sudden changes in the environment, including exposure to heat and oxidants. …

Functional genomic strategies have become fundamental for annotating gene function and
regulatory networks. Here, we combined functional genomics with proteomics by quantifying …

Advances in our ability to systematically introduce and track controlled genetic variance in
microorganisms have, in the past decade, fuelled high-throughput reverse genetics …

10.7554/eLife.31035.001 Understanding how genetic variation contributes to phenotypic
differences is a fundamental question in biology. Combining high-throughput gene function …

All biosynthetically active cells release metabolites, in part due to membrane leakage and
cell lysis, but also in part due to overflow metabolism and ATP-dependent membrane export. …

Lactococcus lactis cheese starter cultures typically contain a mix of many strains and may
include variants that produce and/or tolerate the antimicrobial bacteriocin nisin. Nisin is well-…