Short-read high-throughput RNA sequencing, though powerful, is limited in its ability to
directly measure exon connectivity in mRNAs that contain multiple alternative exons located …

Appreciating the full spectrum of fibroblast heterogeneity in pancreatic ductal adenocarcinoma
is crucial to developing therapies that specifically target tumor-promoting CAFs. This work …

Genome-wide association studies (GWASs) and functional genomics approaches implicate
enhancer disruption in islet dysfunction and type 2 diabetes (T2D) risk. We applied genetic …

Characterization of the cardiac cellulome, the network of cells that form the heart, is essential
for understanding cardiac development and normal organ function and for formulating …

Blood glucose levels are tightly controlled by the coordinated action of at least four cell types
constituting pancreatic islets. Changes in the proportion and/or function of these cells are …

Patients with systemic lupus erythematosus (SLE) display a complex blood transcriptome
whose cellular origin is poorly resolved. Using single-cell RNA sequencing, we profiled ~…

Retinal ganglion cells (RGCs) convey the major output of information collected from the eye
to the brain. Thirty subtypes of RGCs have been identified to date. Here, we analyze 6225 …

The lateral hypothalamic area (LHA) coordinates an array of fundamental behaviors, including
sleeping, waking, feeding, stress and motivated behavior. The wide spectrum of functions …

The natural history of small-cell lung cancer (SCLC) includes rapid evolution from chemosensitivity
to chemoresistance, although mechanisms underlying this evolution remain obscure …

Recursive splicing is a process in which large introns are removed in multiple steps by re-splicing
at ratchet points—5′ splice sites recreated after splicing 1 . Recursive splicing was …