Sequence logos have become a crucial visualization method for studying underlying sequence
patterns in the genome. Despite this, there remains a scarcity of software packages that …

Background Genome-scale CRISPR interference (CRISPRi) has been used in human cell
lines; however, the features of effective guide RNAs (gRNAs) in different organisms have not …

Somatic mutations in cancer genomes include drivers that provide selective advantages to
tumor cells and passengers present due to genome instability. Discovery of pan-cancer …

Screening genome-wide sets of mutants for fitness defects provides a simple but powerful
approach for exploring gene function, mapping genetic networks and probing mechanisms of …

Large-scale proteomic analyses in Escherichia coli have documented the composition and
physical relationships of multiprotein complexes, but not their functional organization into …

Colony-based screens that quantify the fitness of clonal populations on solid agar plates are
perhaps the most important source of genome-scale functional information in …

The effect of single nucleotide variants ( SNV s) in coding and noncoding regions is of great
interest in genetics. Although many computational methods aim to elucidate the effects of …

The pathogenic species of Cryptococcus are a major cause of mortality owing to severe
infections in immunocompromised as well as immunocompetent individuals. Although antifungal …

Protein phosphorylation is important in cellular pathways and altered in disease. We developed
MIMP ( http://mimp.baderlab.org/ ), a machine learning method to predict the impact of …

Interpreting the impact of human genome variation on phenotype is challenging. The
functional effect of protein-coding variants is often predicted using sequence conservation and …