E3 ligases confer specificity to ubiquitination by recognizing target substrates and mediating
transfer of ubiquitin from an E2 ubiquitin-conjugating enzyme to substrate. The activity of …

Cullin–RING complexes comprise the largest known class of ubiquitin ligases. Owing to the
great diversity of their substrate-receptor subunits, it is possible that there are hundreds of …

Oscillations in the activity of cyclin-dependent kinases (CDKs) promote progression through
the eukaryotic cell cycle. This review examines how proteolysis regulates CDK activity—by …

The intracellular levels of many proteins are regulated by ubiquitin-dependent proteolysis.
One of the best-characterized enzymes that catalyzes the attachment of ubiquitin to proteins is …

Depletion of a subset of 7OK stress proteins in yeast mutants shows that they are involved
in the post-translational import of precursor polypeptides into both mitochondria and the …

The 26S proteasome mediates degradation of ubiquitin-conjugated proteins. Although
ubiquitin is recycled from proteasome substrates, the molecular basis of deubiquitination at the …

… In work to be published elsewhere (Reynard and Deshaies, unpublished data), we
demonstrated that Cln2p/Cdc28p protein kinase activity is dependent upon the budding yeast p13 …

Exit from mitosis in budding yeast requires a group of essential proteins—including the GTPase
Tem1 and the protein phosphatase Cdc14—that downregulate cyclin-dependent kinase …

COP9 signalosome (CSN) cleaves the ubiquitin-like protein Nedd8 from the Cul1 subunit of
SCF ubiquitin ligases. The Jab1/MPN domain metalloenzyme (JAMM) motif in the Jab1/…

SCF ubiquitin ligases control various processes by marking regulatory proteins for ubiquitin-dependent
proteolysis. To illuminate how SCF complexes are regulated, we sought proteins …