Heat-shock transcription factor 1 (HSF1) orchestrates the fast and vast cellular response to
heat shock through increased expression of heat-shock proteins. However, how HSF1 rapidly …

Visualization of chromosomal dynamics is important for understanding many fundamental
intra-nuclear processes. Efficient and reliable live-cell multicolor labeling of chromosomal loci …

Gene expression in response to stimuli underlies many fundamental processes. However,
how transcription is regulated under these scenarios is largely unknown. Here, we find a …

The eukaryotic genome is folded into higher-order conformation accompanied with
constrained dynamics for coordinated genome functions. However, the molecular machinery …

Cells sense and respond to extracellular mechanical cues through cell-matrix adhesions.
Interestingly, the maturation of focal adhesions (FAs) is reciprocally force dependent. How …

The CRISPR/Cas9 system has made significant contributions to genome editing, gene
regulation and chromatin studies in recent years. High-throughput and systematic investigations …

Many critical processes occurring in mammalian cells are stochastic and can be directly
observed at the single-molecule level within their physiological environment, which would …

Bacterial binary cell division requires accurate placement of division machinery. FtsZ, a vital
component of the division machinery, can assemble into filaments and self-organize into a …

Protein-protein interactions (PPIs) are critical for cellular activity regulation. Visualization of
PPIs using bimolecular fluorescence complementation (BiFC) techniques helps to …

With the advent of the post-genome era, omics technologies have developed rapidly and
are widely used, including in genomics, transcriptomics, proteomics, metabolomics, and …