Glycosylation plays important roles in cellular function and endows protein therapeutics with
beneficial properties. However, constructing biosynthetic pathways to study and engineer …

Glycosylation is an abundant post-translational modification that is important in disease and
biotechnology. Current methods to understand and engineer glycosylation cannot …

Protein glycosylation, the enzymatic modification of amino acid sidechains with sugar
moieties, plays critical roles in cellular function, human health, and biotechnology. However, …

Protein glycosylation, or the attachment of sugar moieties (glycans) to proteins, is important
for protein stability, activity, and immunogenicity. However, understanding the roles and …

Protein glycosylation, the attachment of sugars to amino acid side chains, can endow proteins
with a wide variety of properties of great interest to the engineering biology community. …

Antibody discovery is bottlenecked by the individual expression and evaluation of antigen-specific
hits. Here, we address this bottleneck by developing a workflow combining cell-free …

Mucin-type-O-glycosylation on proteins is integrally involved in human health and disease
and is coordinated by an enzyme family of 20 N-acetylgalactosaminyltransferases (GalNAc-Ts…

Colicins are antimicrobial proteins produced by Escherichia coli, which, upon secretion from
the host, kill non-host E. coli strains by forming pores in the inner membrane and degrading …

Protein glycosylation is a common post-translational modification that influences the functions
and properties of proteins. Despite advances in methods to produce defined glycoproteins …

Scale‐up of agitated drying processes to minimize particle size changes in active pharmaceutical
ingredients (API) can be challenging. Particle agglomeration or attrition problems due …