… the particular formin and the concentration of profilin (Kovar et al., unpublished). At optimal
… ADP–actin in both the absence and presence of profilin (Kovar et al., unpublished). It is also …

Many aspects of cytoskeletal assembly and dynamics can be recapitulated in vitro; yet, how
the cytoskeleton integrates signals in vivo across cellular membranes is far less understood. …

Cells precisely control the formation of dynamic actin cytoskeleton networks to coordinate
fundamental processes, including motility, division, endocytosis and polarization. To support …

Formin proteins nucleate actin filaments, remaining processively associated with the fast-growing
barbed ends. Although formins possess common features, the diversity of functions …

Formins are large multidomain proteins required for assembly of actin cables that contribute
to the polarity and division of animal and fungal cells. Formin homology-1 (FH1) domains …

… The concentration of profilin is roughly equimolar to the total actin in maize pollen, 125
μM, and the apparent K d of native pollen profilin binding to G-actin is 0.9 μM (DR Kovar, BK …

Microscopy of fluorescent fusion proteins and genetic dependencies show that fission yeast
assemble and constrict a cytokinetic contractile ring in a precisely timed, sequential order. …

Cytokinesis in most eukaryotes requires the assembly and contraction of a ring of actin
filaments and myosin II. The fission yeast Schizosaccharomyces pombe requires the formin …

Formins stimulate actin filament assembly for fundamental cellular processes including division,
adhesion, establishing polarity, and motility. A formin inhibitor would be useful because …

We observed live fission yeast expressing pairs of functional fluorescent fusion proteins to
test the popular model that the cytokinetic contractile ring assembles from a single myosin II …