[HTML][HTML] Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics

SE Ong, B Blagoev, I Kratchmarova… - Molecular & cellular …, 2002 - ASBMB
Quantitative proteomics has traditionally been performed by two-dimensional gel electrophoresis,
but recently, mass spectrometric methods based on stable isotope quantitation have …

Specificity in Toll-like receptor signalling through distinct effector functions of TRAF3 and TRAF6

H Häcker, V Redecke, B Blagoev, I Kratchmarova… - Nature, 2006 - nature.com
Toll-like receptors (TLRs) are activated by pathogen-associated molecular patterns to induce
innate immune responses and production of pro-inflammatory cytokines, interferons and …

A proteomics strategy to elucidate functional protein-protein interactions applied to EGF signaling

B Blagoev, I Kratchmarova, SE Ong, M Nielsen… - Nature …, 2003 - nature.com
Mass spectrometry–based proteomics 1 can reveal protein-protein interactions on a large
scale 2 , 3 , but it has been difficult to separate background binding from functionally important …

Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics

B Blagoev, SE Ong, I Kratchmarova, M Mann - Nature biotechnology, 2004 - nature.com
To study the global dynamics of phosphotyrosine-based signaling events in early growth
factor stimulation, we developed a mass spectrometric method that converts temporal changes …

Mechanism of divergent growth factor effects in mesenchymal stem cell differentiation

I Kratchmarova, B Blagoev, M Haack-Sorensen… - Science, 2005 - science.org
Closely related signals often lead to very different cellular outcomes. We found that the
differentiation of human mesenchymal stem cells into bone-forming cells is stimulated by …

Properties of 13C-Substituted Arginine in Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)

SE Ong, I Kratchmarova, M Mann - Journal of proteome research, 2003 - ACS Publications
We have recently described a method, stable isotope labeling by amino acids in cell culture
(SILAC) for the accurate quantitation of relative protein abundances. Cells were …

System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation

…, J Henningsen, PT Johansen, I Kratchmarova… - Science …, 2011 - science.org
To elucidate cellular events underlying the pluripotency of human embryonic stem cells (hESCs),
we performed parallel quantitative proteomic and phosphoproteomic analyses of …

UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites

…, JT Vanselow, MM Nielsen, I Kratchmarova… - Nature structural & …, 2018 - nature.com
Ubiquitination is a post-translational modification (PTM) that is essential for balancing
numerous physiological processes. To enable delineation of protein ubiquitination at a site-…

[HTML][HTML] Dynamics of the skeletal muscle secretome during myoblast differentiation

…, B Blagoev, BK Pedersen, I Kratchmarova - Molecular & cellular …, 2010 - ASBMB
During recent years, increased efforts have focused on elucidating the secretory function of
skeletal muscle. Through secreted molecules, skeletal muscle affects local muscle biology in …

[HTML][HTML] A proteomic approach for identification of secreted proteins during the differentiation of 3T3-L1 preadipocytes to adipocytes

I Kratchmarova, DE Kalume, B Blagoev… - Molecular & Cellular …, 2002 - ASBMB
We have undertaken a systematic proteomic approach to purify and identify secreted factors
that are differentially expressed in preadipocytes versus adipocytes. Using one-dimensional …