Protein glycosylation, the enzymatic modification of amino acid sidechains with sugar
moieties, plays critical roles in cellular function, human health, and biotechnology. However, …

The advent of distributed biomanufacturing platforms promises to increase agility in biologic
production and expand access by reducing reliance on refrigerated supply chains. However…

Conjugate vaccines are among the most effective methods for preventing bacterial infections.
However, existing manufacturing approaches limit access to conjugate vaccines due to …

Protein glycosylation, or the attachment of sugar moieties (glycans) to proteins, is important
for protein stability, activity, and immunogenicity. However, understanding the roles and …

Cell-free gene expression (CFE) systems from crude cellular extracts have attracted much
attention for biomanufacturing and synthetic biology. However, activating membrane-…

Particulate methane monooxygenase (pMMO) is a multi-subunit membrane metalloenzyme
used by methanotrophic bacteria to convert methane to methanol. A major hurdle to studying …

High-throughput quantification of the post-translational modification of many individual protein
samples is challenging with current label-based methods. This paper demonstrates an …

Bacterial microcompartments (MCPs) are protein-based organelles that encapsulate metabolic
pathways. Metabolic engineers have recently sought to repurpose MCPs to encapsulate …

Structural proteins such as “suckerins” present promising avenues for fabricating functional
materials. Suckerins are a family of naturally occurring block copolymer-type proteins that …

Asparagine-linked (N-linked) protein glycosylation is one of the most abundant types of
posttranslational modification, occurring in all domains of life. The central enzyme in N-linked …