User profiles for J. Hershewe
Jasmine HershewePh.D. Candidate, Northwestern University Verified email at u.northwestern.edu Cited by 345 |
Cell-free systems for accelerating glycoprotein expression and biomanufacturing
J Hershewe, W Kightlinger… - Journal of Industrial …, 2020 - academic.oup.com
Protein glycosylation, the enzymatic modification of amino acid sidechains with sugar
moieties, plays critical roles in cellular function, human health, and biotechnology. However, …
moieties, plays critical roles in cellular function, human health, and biotechnology. However, …
Rapid biosynthesis of glycoprotein therapeutics and vaccines from freeze-dried bacterial cell lysates
The advent of distributed biomanufacturing platforms promises to increase agility in biologic
production and expand access by reducing reliance on refrigerated supply chains. However…
production and expand access by reducing reliance on refrigerated supply chains. However…
On-demand biomanufacturing of protective conjugate vaccines
Conjugate vaccines are among the most effective methods for preventing bacterial infections.
However, existing manufacturing approaches limit access to conjugate vaccines due to …
However, existing manufacturing approaches limit access to conjugate vaccines due to …
A cell‐free platform for rapid synthesis and testing of active oligosaccharyltransferases
Protein glycosylation, or the attachment of sugar moieties (glycans) to proteins, is important
for protein stability, activity, and immunogenicity. However, understanding the roles and …
for protein stability, activity, and immunogenicity. However, understanding the roles and …
[HTML][HTML] Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles
Cell-free gene expression (CFE) systems from crude cellular extracts have attracted much
attention for biomanufacturing and synthetic biology. However, activating membrane-…
attention for biomanufacturing and synthetic biology. However, activating membrane-…
Cell-free protein synthesis of particulate methane monooxygenase into nanodiscs
Particulate methane monooxygenase (pMMO) is a multi-subunit membrane metalloenzyme
used by methanotrophic bacteria to convert methane to methanol. A major hurdle to studying …
used by methanotrophic bacteria to convert methane to methanol. A major hurdle to studying …
High-throughput synthesis and analysis of intact glycoproteins using SAMDI-MS
High-throughput quantification of the post-translational modification of many individual protein
samples is challenging with current label-based methods. This paper demonstrates an …
samples is challenging with current label-based methods. This paper demonstrates an …
[HTML][HTML] Apparent size and morphology of bacterial microcompartments varies with technique
Bacterial microcompartments (MCPs) are protein-based organelles that encapsulate metabolic
pathways. Metabolic engineers have recently sought to repurpose MCPs to encapsulate …
pathways. Metabolic engineers have recently sought to repurpose MCPs to encapsulate …
Characterizing and controlling nanoscale self-assembly of suckerin-12
JM Hershewe, WD Wiseman, JE Kath… - ACS synthetic …, 2020 - ACS Publications
Structural proteins such as “suckerins” present promising avenues for fabricating functional
materials. Suckerins are a family of naturally occurring block copolymer-type proteins that …
materials. Suckerins are a family of naturally occurring block copolymer-type proteins that …
A pipeline for studying and engineering single-subunit oligosaccharyltransferases
Asparagine-linked (N-linked) protein glycosylation is one of the most abundant types of
posttranslational modification, occurring in all domains of life. The central enzyme in N-linked …
posttranslational modification, occurring in all domains of life. The central enzyme in N-linked …