User profiles for M. C. Jewett
Michael C. JewettStanford University Verified email at stanford.edu Cited by 16281 |
Cell-free gene expression: an expanded repertoire of applications
Cell-free biology is the activation of biological processes without the use of intact living cells.
It has been used for more than 50 years across the life sciences as a foundational research …
It has been used for more than 50 years across the life sciences as a foundational research …
[HTML][HTML] Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery
The emerging discipline of bacterial glycoengineering has made it possible to produce
designer glycans and glycoconjugates for use as vaccines and therapeutics. Unfortunately, cell-…
designer glycans and glycoconjugates for use as vaccines and therapeutics. Unfortunately, cell-…
[HTML][HTML] High-throughput preparation methods of crude extract for robust cell-free protein synthesis
Crude extract based cell-free protein synthesis (CFPS) has emerged as a powerful
technology platform for high-throughput protein production and genetic part characterization. …
technology platform for high-throughput protein production and genetic part characterization. …
Precise manipulation of chromosomes in vivo enables genome-wide codon replacement
We present genome engineering technologies that are capable of fundamentally reengineering
genomes from the nucleotide to the megabase scale. We used multiplex automated …
genomes from the nucleotide to the megabase scale. We used multiplex automated …
[HTML][HTML] Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids
… To make cell extract, the thawed cells were suspended in 0.8 mL of S30 buffer per 1 g of
wet cell mass and processed as reported by Kwon and Jewett 59 . Full details for cell growth, …
wet cell mass and processed as reported by Kwon and Jewett 59 . Full details for cell growth, …
Mimicking the Escherichia coli cytoplasmic environment activates long‐lived and efficient cell‐free protein synthesis
MC Jewett, JR Swartz - Biotechnology and bioengineering, 2004 - Wiley Online Library
… This is not possible with conventional secondary energy sources such as PEP, which
eventually poison the system (Jewett and Swartz, in press). Stability of pH is another attractive …
eventually poison the system (Jewett and Swartz, in press). Stability of pH is another attractive …
Cell-free protein synthesis: applications come of age
Cell-free protein synthesis has emerged as a powerful technology platform to help satisfy
the growing demand for simple and efficient protein production. While used for decades as a …
the growing demand for simple and efficient protein production. While used for decades as a …
How many human proteoforms are there?
Despite decades of accumulated knowledge about proteins and their post-translational
modifications (PTMs), numerous questions remain regarding their molecular composition and …
modifications (PTMs), numerous questions remain regarding their molecular composition and …
Cell-free synthetic biology: thinking outside the cell
CE Hodgman, MC Jewett - Metabolic engineering, 2012 - Elsevier
Cell-free synthetic biology is emerging as a powerful approach aimed to understand, harness,
and expand the capabilities of natural biological systems without using intact cells. Cell-…
and expand the capabilities of natural biological systems without using intact cells. Cell-…
Cell‐free metabolic engineering: Biomanufacturing beyond the cell
… For example, Jewett et al. demonstrated that formation of inverted lipid vesicles during extract
preparation facilitates oxidative phosphorylation which converts reducing equivalents from …
preparation facilitates oxidative phosphorylation which converts reducing equivalents from …