The astonishing progress made during the last two decades toward understanding the
machinery of cells has been fueled in large part by developments in structural biology, molecular …

… However, injection of taxol into stage 11 oocytes has no apparent impact on fast streaming,
suggesting that microtubule dynamics are not necessary to drive the flows (ME Quinlan, …

The structural change that generates force and motion in actomyosin motility has been
proposed to be tilting of the myosin light chain domain, which serves as a lever arm. Several …

Several complementary techniques have been developed to determine average orientation,
dynamics on multiple time scales, and concerted rotational motions of individual fluorescent …

… Ca-actin was converted to Mg-actin by incubation in ME (50 mM MgCl 2 , 0.2 mM EGTA)
for 2 min before addition of KMEI and test components. Unless otherwise indicated, 0.5 µM …

Many cellular structures are assembled from networks of actin filaments, and the architecture
of these networks depends on the mechanism by which the filaments are formed. Several …

Spire and Cappuccino are actin nucleation factors that are required to establish the polarity
of Drosophila melanogaster oocytes. Their mutant phenotypes are nearly identical, and the …

A new approach is presented for measuring the three-dimensional orientation of individual
macromolecules using single molecule fluorescence polarization (SMFP) microscopy. The …

Evidence for cooperation between actin nucleators is growing. The WH2-containing nucleator
Spire and the formin Cappuccino interact directly, and both are essential for assembly of …

The Gram-positive bacterium Listeria monocytogenes is a facultative intracellular pathogen
whose virulence depends on its ability to spread from cell to cell within an infected host. …