We use an oligonucleotide library of >10,000 variants to identify an insulation mechanism
encoded within a subset of σ 54 promoters. Insulation manifests itself as reduced protein …

We explore a model for ‘quenching-like’ repression by studying synthetic bacterial enhancers,
each characterized by a different binding site architecture. To do so, we take a three-…

Whole cell bioreporters, such as bacterial cells, can be used for environmental and clinical
sensing of specific analytes. However, the current methods implemented to observe such …

Chemotaxis is the movement of an organism in response to an external chemical stimulus.
This system enables bacteria to sense their immediate environment and adapt to changes in …

We present Triplex-seq, a deep-sequencing method that systematically maps the interaction
space between an oligo library of ssDNA triplex-forming oligos (TFOs) and a particular …

… Author links open overlay panel Nancy M Kim 1 , Riley W Sinnott 2 , Nicholas R
Sandoval 2 … 0.1–10 μmol/m 2 s 1 … Castillo-Hair, M. Fujita, OA Igoshin, JJ Tabor …

Nucleoprotein complexes play an integral role in genome organization of both eukaryotes
and prokaryotes. Apart from their role in locally structuring and compacting DNA, several …

With new advances in infectious disease, antifouling surfaces, and environmental microbiology
research comes the need to understand and control the accumulation and attachment of …

In 1961, Jacob and Monod proposed the operon model of gene regulation. At the model's
core was the modular assembly of regulators, operators, and structural genes. To illustrate the …

We study translation repression in bacteria by engineering a regulatory circuit that functions
as a binding assay for RNA binding proteins (RBP) in vivo. We do so by inducing expression …