The β subunit of the Escherichia coli replicative DNA polymerase III holoenzyme is the sliding
clamp that interacts with the α (polymerase) subunit to maintain the high processivity of the …

A synthetic Synechocystis sp. PCC6803 DnaB split mini-intein gene was constructed for the
in vivocyclization of recombinant proteins expressed in Escherichia coli. The system was …

The sliding clamp of the Escherichia coli replisome is now understood to interact with many
proteins involved in DNA synthesis and repair. A universal interaction motif is proposed to be …

The initial contact between axons and dendrites at early neuronal synapses is mediated by
surface adhesion molecules and is thought to induce synaptic maturation through the …

The bacterial replisome is a target for the development of new antibiotics to combat drug
resistant strains. The β 2 sliding clamp is an essential component of the replicative machinery, …

Abstract Changes in protein conformation are thought to alter charge state distributions
observed in electrospray ionization mass spectra (ESI-MS) of proteins. In most cases, this has …

… Similar to WT mice, both netrin-G1 KO and netrin-G2 KO mice remained in the platform region
for a significantly longer time than in the visual cliff zone (p < 0.0001 for all groups, Student’…

A mutant version of the N-terminal domain of Escherichia coli DnaB helicase was used as a
model system to assess the stabilization against unfolding gained by covalent cyclization. …

… However, we found that use of tandem phage λ p R and p L promoters for expression did
permit construction of stably transformed strains. The λ-promoter plasmid pKL1176 (Fig. 1) …

… This was also a major obstacle in our ptitification efforts with the pea enzyme (PV Prosselkov
and W. Gross, unpublished data). In contrast, the enzyme from Galdieria sulphuraria proved …