The trafficking of proteins within eukaryotic cells is achieved by the capture of cargo and
targeting molecules into vesicles that bud from a donor membrane and deliver their contents to …

… The enrichment procedure was first performed on an a strain (NFl R) and then on an (Y
strain (SF 1823B). The set mutants were arranged into complementatjon groups by standard …

In vitro synthesis of endoplasmic reticulum-derived transpofl vesicles has been reconstituted
with washed membranes and three soluble proteins (Sarl p, Secl3p complex, and Sec23p …

▪ Abstract The endoplasmic reticulum (ER) and the Golgi comprise the first two steps in
protein secretion. Vesicular carriers mediate a continuous flux of proteins and lipids between …

… with lyticase (Scott and Schekman, 19801, and extracts were prepared by detergent lysis.
Labeled invertase … Dried gels were allowed to expose Kodak X-Omat R film XR-5 for 1-2 days. …

COPII vesicle formation requires only three coat assembly subunits: Sar1p, Sec13/31p, and
Sec23/24p. PI 4-phosphate or PI 4,5-bisphosphate is required for the binding of these …

Subcellular compartments have unique protein compositions, yet protein synthesis only
occurs in the cytosol and in mitochondria and chloroplasts. How do proteins get where they …

We have characterized the mechanisms of cargo selection into ER-derived vesicles by the
COPII subunit Sec24p. We identified a site on Sec24p that recognizes the v-SNARE Bet1p …

… Substitution of the charged residues (D 5 and R 11 D 12 ) with alanine had little impact on
the membrane binding or coat-recruitment ability of Sar1p (Figure 5B). However, substitution …

… to the Golgi apparatus (C, Kaiser and R. Schekman, unpublished observation) and among
mutants affecting protein delivery to the cell surface (Salminen and Novick, 1987). In each …